ASTar kits

An ASTar kit contains two disposables, a sample preparation cartridge and an AST disc.

Cartridge

The cartridge contains all reagents and disposable articles needed for sample preparation, concentration determination, dilution and growth medium adaptation.

Contains pre-deposited reagents in a combination of freeze-dried and room-temperature liquids. A frozen insert is added to the cartridge before use
Filters millimetre-sized resin composites present in many types of blood culture bottles and otherwise risk interfering with the analysis
Has barcodes for identifying and linking the cartridge and patient sample
Stored at room temperature

AST disc

The AST disc is used for AST and concentration determination.

Contains more than 330 culturing chambers with prefilled antimicrobials in various concentrations used for AST, chambers without antimicrobials used as controls, and chambers used to determine the concentration in the added sample
Contains unique barcode for identification and linking to each respective sample preparation cartridge and patient
Stored at room temperature

Cartridge

The cartridge contains all reagents and disposable articles needed for sample preparation, concentration determination, dilution and growth medium adaptation.

Contains pre-deposited reagents in a combination of freeze-dried and room-temperature liquids. A frozen insert is added to the cartridge before use
Filters millimetre-sized resin composites present in many types of blood culture bottles and otherwise risk interfering with the analysis
Has barcodes for identifying and linking the cartridge and patient sample
Stored at room temperature

AST disc

The AST disc is used for AST and concentration determination.

Contains more than 330 culturing chambers with prefilled antimicrobials in various concentrations used for AST, chambers without antimicrobials used as controls, and chambers used to determine the concentration in the added sample
Contains unique barcode for identification and linking to each respective sample preparation cartridge and patient
Stored at room temperature

ASTar BC G- panel

Preliminary panel for AST analysis of gram-negative bacteria (including fastidious pathogens) from positive blood cultures.

Antimicrobial class	Antimicrobial agent	Range (mg/L) Non-Fastidious	Range (mg/L) Fastidious	# Dilutions
Aminoglycoside	Amikacin	0.5-128		9
Aminoglycoside	Gentamicin	0.25-32		8
Aminoglycoside	Tobramycin	0.125-32		9
Carbapenem	Ertapenem	0.015-4		9
Carbapenem	Imipenem	0.03-16		10
Carbapenem	Meropenem	0.125-64		10
Carbapenem	Meropenem-vaboractam	0.06-32		10
Cephalosporin	Cefazolin	0.25-16		7
Cephalosporin	Cefepime	0.25-64		9
Cephalosporin	Cefotaxime	0.015-128		14
Cephalosporin	Cefoxitin	1-64		7
Cephalosporin	Ceftazidime	0.25-64		9
Cephalosporin	Ceftazidime-avibactam	0.25-32		8
Cephalosporin	Ceftolozane-tazobactam	0.125-32		9
Cephalosporin	Ceftriaxone	0.015-128		14
Cephalosporin	Cefuroxime	1-64		7
Fluoroquinolone	Ciprofloxacin	0.015-8		10
Fluoroquinolone	Levofloxacin	0.125-16		8
Miscellaneous	Trimethoprim-sulfamethoxazole	0.06-16		9
Monobactam	Aztreonam	0.25-64		9
Penicillin	Amoxicillin-clavulanic acid	1-32		6
Penicillin	Ampicillin	1-64		7
Penicillin	Ampicillin-sulbactam EU	0.5-128		9
Penicillin	Ampicillin-sulbactam US	1-64		7
Penicillin	Piperacillin-tazobactam	0.5-256		10
Polymyxin	Colistin	0.25-8		6
Tetracycline	Tigecycline	0.015-16		11
Carbapenem	Meropenem		0.03-8	9
Cephalosporin	Cefotaxime		0.015-2	8
Cephalosporin	Ceftriaxone		0.015-2	8
Fluoroquinolone	Levofloxacin		0.015-2	8
Penicillin	Amoxicillin-clavulanic acid		0.5-32	7
Penicillin	Ampicillin		0.25-8	6

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Flexible solution enables future panel extensions

ASTar system can be extended to other types of clinical samples. The modular design of the sample preparation cartridge, in combination with the AST disc, allows for future adaptation to other sample types such as isolates, urine, respiratory and sterile aspirates.